Chromium Single Cell

Universal 3'

GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species

Up to 80% cell recovery

Up to 100% higher sensitivity vs prior Next GEM tech

More usable reads, save up to 50% on sequencing costs

Why choose Universal 3'?

  • Require full-length transcript readouts

  • Flexible throughput from 500 to 20K cells per sample

  • Broad multiomic options including protein and gene expression

Assay specifications

    Species

  • Human

  • Mouse

  • Rat

  • Non-human primate

  • Marine

  • Plant

  • Insect

  • and more

    Compatible samples

  • Cell suspension

  • Nuclei suspension

  • Fresh tissue

  • Frozen tissue

  • Flow-sorted cells

  • Fixed human PBMCs

  • Organoids

    Multiomics

  • 3' gene expression

  • Cell surface protein

    Singleplex throughput options

  • 20K cells/sample

  • 160K cells/chip

  • 8 samples/chip

Shop reagents, kits & more

Chromium Single Cell

Universal 3' Gene Expression

3' User Guides

Each 3' experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. If you're unsure what you need, use the experiment builder to find the custom list of items required for your experiment.

Version
Select version
Compatible instruments

Build your own Universal 3' experiment

Design the experiment that's right for you

Documents and resources

Videos

Cell Ranger image

Cell Ranger

Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data.

Loupe Browser image

Loupe Browser

Desktop application for Windows and MacOS that allows you to interactively visualize data.

Cloud Analysis image

Cloud Analysis

Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser.

Featured publications

Featured datasets

Flex Apex

Probe-based whole transcriptome profiling for fresh, frozen, or fixed samples (even FFPE), plus 96-well multiplexing for highest throughput.

Universal 5'

5' gene expression alongside V(D)J repertoire profiling and antigen specificity of T and B cells.

Epi Multiome ATAC + Gene Expression

Combined profiling of 3' gene expression and chromatin accessibility from the same cell.

Epi ATAC

Chromatin accessibilility profiling at the single cell level.

Chromium Single Cell

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