Chromium Single Cell
Universal 3' Gene Expression
GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species
Up to 80% cell recovery
Up to 100% higher sensitivity vs prior Next GEM tech
More usable reads, save up to 50% on sequencing costs
Why choose Universal 3' Gene Expression
Assay specifications
Species
Mouse
Human
Rat
Non-human primate
Marine
Plant
Insect
and moreCompatible samples
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
Flow-sorted cells
Fixed human PBMCs
OrganoidsMultiomics
3' gene expression
Cell surface proteinCompatible instruments
Throughput options
Learn moreSingleplex
20K cells/sample
160K cells/chip
8 samples/chipOn-chip multiplexing (New!)
5K cells/sample40K cells/chipUp to 4 samples/channel output, 8 samples/chipComponents
Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. If you're unsure what you need, use the experiment builder find the custom list of items required for your experiment.
Consumables
Choose a version
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Chip
1000747
Kit, GEM-X OCM 3' Chip Kit v4 4-plex, 2 chips
Index Kit
1000215
Dual Index Kit TT Set A 96 rxns
1000242
Dual Index Kit NT Set A, 96 rxn
Core Reagents
1000779
GEM-X Universal 3’ Gene Expression v4 4-plex, 16 samples
Library Construction Kit (optional)
1000694
Library Construction Kit C, 16 rxns
Feature Barcoding Kit (optional)
1000702
Chromium GEM-X Single Cell 3 Feature Barcode Kit v4, 16 rxns
Build your own Universal 3' Gene Expression experiment
Design the experiment that's right for you
Documents and resources
Product sheet
User guides
Sequencing requirements
Universal 3’ Gene Expression produces Illumina® sequencer-ready libraries. Minimum 20,000 read pairs/cell
View allVideos
Chromium GEM-X: See the next generation of single cell in action
Unlocking cellular secrets: The powerful role of single cell analysis in cell therapy and other clinical applications
Single Cell Gene Expression: Practical considerations and best practices for getting started
Data analysis
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Learn more
Loupe Browser
Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data
Learn more
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Learn moreFeatured publications
Single-cell atlas reveals correlates of high cognitive function, dementia, and resilience to Alzheimer’s disease pathology
Cell, 2023
Hansruedi Mathys, et al.
An autocrine signaling circuit in hepatic stellate cells underlies advanced fibrosis in nonalcoholic steatohepatitis
Science Translational Medicine, 2023
Shuang Wang, et al.
Single-cell RNA-seq reveals cell type–specific molecular and genetic associations to lupus
Science, 2022
Richard K Perez, et al.
Featured datasets
10k Human DTC Melanoma, Chromium GEM-X Single Cell 3'
View dataset10k Human PBMCs Stained with TotalSeq™-B Human TBNK Cocktail, Chromium GEM-X Single Cell 3'
View dataset10k Adult Mouse Brain Nuclei Isolated with Chromium Nuclei Isolation Kit, Chromium GEM-X Single Cell 3'
View datasetRelated products
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Epi Multiome ATAC + Gene Expression
Single cell profiling of open chromatin and 3' gene expression from the same nucleus
Epi ATAC
Single cell profiling of open chromatin