Chromium Single Cell
Epi Multiome ATAC + Gene Expression
Single cell profiling of open chromatin and 3' gene expression from the same nucleus
10K nuclei/sample or 80K nuclei/chip
Run 8 samples in parallel
Why choose Epi Multiome ATAC + Gene Expression
Assay specifications
Species
Mouse
Human
Rat
Non-human primate
Marine
Plant
InsectCompatible samples
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
OrganoidsMultiomics
Chromatin accessibility
3' gene expressionCompatible instruments
Throughput options
Singleplex
10K nuclei/sample
80K nuclei/chip
8 samples/chipAssay Components
Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. If you’re unsure what you need, use the experiment builder find the custom list of items required for your experiment.
Consumables
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Chip
1000230
Chromium Next GEM Chip J Single Cell Kit, 16 rxns
1000234
Chromium Next GEM Chip J Single Cell Kit, 48 rxns
Index Kit
1000215
Dual Index Kit TT Set A 96 rxns
1000212
Single Index Kit N Set A, 96 rxn
Core Reagents
1000285
Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns
1000283
Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns
Documents and resources
Product sheet
User guides
Sequencing requirements
Minimum 20,000 read pairs/nucleus for gene expression; 25,000 read pairs per nucleus for ATAC
View allAnalysis guides
Videos
How it works
Simultaneous profiling of the transcriptome and epigenome at single cell resolution
Revealing the Developing Brain With Single-Cell Multiomic Profiling of Human Cerebral Organoids
Highly multimodal measurements of single cells
Deep Immune Profiling at Scale: Efficient Pipelines and TEA-seq for Simultaneous Trimodal Measurements
Data analysis
Cell Ranger ARC
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell Multiome ATAC + Gene Expression data
Learn more
Loupe Browser
Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data
Learn more
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Learn moreFeatured publications
Mechanisms of action and resistance in histone methylation-targeted therapy
Nature, 2024
Makoto Yamagishi, et al.
Single-cell systems pharmacology identifies development-driven drug response and combination therapy in B cell acute lymphoblastic leukemia
Cancer Cell, 2024
Xin Huang, et al.
Genetic and epigenetic coordination of cortical interneuron development
Nature, 2021
Kathryn C Allaway, et al.
Inferring and perturbing cell fate regulomes in human brain organoids
Nature, 2022
Jonas Simon Fleck, et al.
Cross-lineage potential of Ascl1 uncovered by comparing diverse reprogramming regulatomes
Cell Stem Cell, 2022
Haofei Wang, et al.
Featured datasets
Multiomic Integration Neuroscience Application Note: Single Cell Multiome RNA + ATAC Alzheimer's Disease Mouse Model Brain Coronal Sections from One Hemisphere Over a Time Course
View datasetHuman Kidney Cancer Nuclei isolated with Chromium Nuclei Isolation Kit, SaltyEZ Protocol, and 10x Complex Tissue DP (CT Sorted and CT Unsorted)
View datasetFlash-Frozen Lymph Node with B Cell Lymphoma (14k sorted nuclei)
View dataset10k Human PBMCs, Multiome v1.0, Chromium X
View datasetFlash-Frozen Human Healthy Brain Tissue (3k)
View datasetRelated products
Flex Gene Expression
Probe-based assays using GEM-X technology to capture 18,000+ coding genes, customizable to fit project needs
Universal 5' Gene Expression
RT-based assays using GEM-X techology to analyze immune diversity
Universal 3' Gene Expression
RT-based assays using GEM-X techology to capture the whole transcriptome to gather broad information from diverse species
Epi ATAC
Single cell profiling of open chromatin