Chromium Single Cell
Flex Gene Expression
GEM-X technology-powered assays with protein coding gene coverage, customizable to fit project needs
Exceptional performance with challenging samples
Reduced cell input recommendations: 25K
Why choose Flex Gene Expression
Assay specifications
Species
Mouse
Human
Other species with custom probesCompatible samples
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
FFPE tissue
PFA-fixed tissue
Flow-sorted cells
Fixed whole blood
OrganoidsMultiomics
Probe-based gene expression
Cell surface protein
Intracellular protein
CRISPR screeningCompatible instruments
Throughput options
Singleplex
20K cells/sample
160K cells/chip
8 samples/chipIn-line multiplexing
20K cells/sample2.56M cells/chip
128 samples/chipComponents
Each experiment requires a chip, index kit, and core reagents. If you're unsure what you need, use the experiment builder find the custom list of items required for your experiment.
Consumables
Choose a version
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Sample Prep Kit
1000781
GEM-X Flex Sample Preparation v2 Kit, 48 rxns
Individual Wash Workflow (optional)
1000828
GEM-X Flex Supplemental Wash Kit, 64 rxns
Chip
1000791
GEM-X Flex Gene Expression Chip Kit, 4 chips
Index Kit
1000251
Dual Index Kit TS Set A, 96 rxn
1000215
Dual Index Kit TT Set A 96 rxns
Core Reagents
1000792
GEM-X Flex Gene Expression Human, 4 samples
1000793
GEM-X Flex Gene Expression Human 4-plex, 16 samples
1000794
GEM-X Flex Gene Expression Human 16-plex, 64 samples
1000829
GEM-X Flex Gene Expression Human n-plex, 64 samples
1000795
GEM-X Flex Gene Expression Human 16-plex, 256 samples
1000796
GEM-X Flex Gene Expression Mouse, 4 samples
1000797
GEM-X Flex Gene Expression Mouse 4-plex, 16 samples
1000798
GEM-X Flex Gene Expression Mouse 16-plex, 64 samples
1000831
GEM-X Flex Gene Expression Mouse n-plex, 64 samples
1000799
GEM-X Flex Gene Expression Mouse 16-plex, 256 samples
Feature Barcoding Kit (optional)
1000628
Fixed RNA Feature Barcode Multiplexing Kit, 64 rxns
Build your own Flex Gene Expression experiment
Design the experiment that's right for you
Documents and resources
Product sheet
User guides
Sequencing requirements
Minimum 10,000 read pairs/cell for gene expression; 5,000 read pairs/cell for protein analysis.
View allDemonstrated protocol
Videos
How it works
Change the rules for scRNA-seq: Access more samples, run on your schedule
Single cell analysis of FFPE tissue: Experiences and considerations from a pathologist’s perspective
Direct fixation of whole blood for downstream single cell analysis
High-throughput single cell profiling with streamlined workflows and multiomic readouts at unparalleled scale
Data analysis
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Learn more
Loupe Browser
Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data
Learn more
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Learn moreFeatured publications
Single-cell systems pharmacology identifies development-driven drug response and combination therapy in B cell acute lymphoblastic leukemia
Cancer Cell, 2024
Xin Huang, et al.
Peroxisome deficiency underlies failures in hepatic immune cell development and antigen presentation in a severe Zellweger disease model
Cell Reports, 2024
Brendon D Parsons, et al.
Cortical somatostatin long-range projection neurons and interneurons exhibit divergent developmental trajectories
Neuron, 2023
Josephine Fisher, et al.
Featured datasets
10k Mouse Forebrain FFPE Tissue Dissociated using gentleMACS Dissociator, Singleplex Sample
View datasetAggregate of ~900k Human Non Small Cell Lung Cancer and Normal Adjacent Cells, Multiplexed Samples, 16 Probe Barcodes
View dataset128k Human PBMCs Stained with TotalSeq™-C Human Universal Cocktail
View datasetHuman Ovarian Cancer FFPE Single Cell Gene Expression Flex
View datasetAggregate of Human PBMC Isolated from Fixed Whole Blood of a Diseased Donor and a Healthy Donor
View datasetRelated products
Universal 3' Gene Expression
GEM-X technology-powered assays with whole transcriptome coverage to gather broad information from diverse species
Universal 5' Gene Expression
GEM-X technology-powered assays with whole transcriptome coverage to analyze immune diversity
Epi Multiome ATAC + Gene Expression
Single cell profiling of open chromatin and 3' gene expression from the same nucleus
Epi ATAC
Single cell profiling of open chromatin