Whole transcriptome gene expression, with ultimate flexibility in sample prep and the ability to fix, batch, and run samples on your schedule
We recommend Flex Gene Expression in experiments that...
Use archival or lower-quality samples
Require flexible sample batching
Will scale up and require the lowest cost per-cell
A versatile solution providing 3' gene expression with options for multiomic analysis
We recommend 3' in experiments that...
Require full-length transcript readouts, with or without simultaneous protein expression
Only require gene expression, or may use ATAC in the future
Have lower cell input requirements
Comprehensive analysis of immune diversity, combining 5' gene expression with multiomic profiling
We recommend Universal 5' in experiments that...
Require full length transcript readouts, with or without simultaneous protein expression
Require or may use CRISPR and TCR/BCR sequencing in the future
Have lower cell input requirements
Primary capability
Probe-based gene expressionAnalyze gene expression using pre-designed probes with the option to add custom probes
3' gene expressionDetect and quantify RNA molecules near the 3' end of genes
5' gene expressionDetect and quantify RNA molecules near the 5' end of genes
Multiomic capabilities
Cell surface proteinIdentify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
CRISPR screeningAssess effects of CRISPR perturbations at single cell resolution with direct capture of cellular sgRNAs and changes in gene expression
Intracellular proteinAchieve detection of proteins throughout the entire cell, including nuclear, cytosolic, and secreted proteins
Cell surface proteinIdentify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
Cell surface proteinIdentify surface protein markers to categorize cell types, pinpoint biomarkers, and explore cell signaling and interactions
CRISPR screeningAssess effects of CRISPR perturbations at single cell resolution with direct capture of cellular sgRNAs and changes in gene expression
TCR/BCR sequencingUnderstand clonal expansion and diversity by capturing full-length, paired B-cell and T-cell receptors
Antigen specificityInterrogate the binding of antigens to immune cell receptors to fully characterize the adaptive immune response.
Compatible species
Compatible sample types
Resolution
Whole cell/NucleusCells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Whole cell/NucleusCells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Whole cell/NucleusCells or nuclei are partitioned into GEM Gel Beads that uniquely barcode each, enabling single cell resolution
Run configuration options
Compatible instruments
Chromium iX and XAccess our core assays and capabilities with the iX or take advantage of our full single cell portfolio with the Chromium X.
Chromium Xo, iX, and XPerform 3' gene expression with the low-cost Xo instrument, access our core assays and capabilities with the iX, or take advantage of our full single cell portfolio with the Chromium X.
Chromium iX and XAccess our core assays and capabilities with the iX or take advantage of our full single cell portfolio with the Chromium X.
Included data processing & analysis software
Cell RangerCell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud AnalysisA platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Cell RangerCell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud AnalysisA platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Cell RangerCell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data on-prem.
Cloud AnalysisA platform for running 10x analysis pipelines on optimized cloud clusters for a quick and easy path to results.
Included interactive visualization tools
Loupe BrowserVisualize and interpret Chromium single cell gene expression data with Loupe Browser, a visualization tool that enables identification of gene expression patterns and cell clusters.
Loupe BrowserVisualize and interpret Chromium single cell gene expression data with Loupe Browser, a visualization tool that enables identification of gene expression patterns and cell clusters.
Loupe BrowserInteractively explore clonotype information and repertoire diversity alongside gene expression data in Loupe Browser.
Loupe V(D)J BrowserInvestigate clonotypes and examine V(D)J transcripts with this interactive visualization desktop software.