1k A375 Cells Transduced with Non-Target and Target sgRNA, Chromium GEM-X Single Cell 5'
Universal 5' Gene Expression dataset analyzed using Cell Ranger 8.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
1K A375 cells, a human cell line derived from malignant melanoma cells, stably expressing dCas9 were separately transduced with single guides (either non-targeting or Rab1a sgRNA).
Gene Expression and CRISPR libraries were generated as described in the Chromium GEM-X Single Cell 5' Reagent Kits v3 User Guide (CG000735) and sequenced on an Illumina NovaSeq6000 with approximately 80,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.