You may edit the panel based on results in the panel design summary as many times as needed. When you are satisfied with the design, select the relevant option to finalize the design on the Review Design Recommendations page (10x support will do this step for the advanced workflow option).
For the standard workflow option, a 6-digit design ID will be generated. The design ID is used to order your first panel, as well as reorders of the same panel in the future. The output files associated with your panel will be generated for download.
On the finalized panel design screen, you can:
- Contact your sales representative with the design ID to request a quote to start the panel ordering process.
- Learn what is included in your panel(s) order
- Upload the panel JSON file to the Xenium Analyzer instrument
- Download panel design output files (described below)
The following files are provided once the panel design is finalized:
| Panel design output files | Description |
|---|---|
| BED file | A file with custom panel gene targets, which can be used to gauge whether a limited number of probes target CDSs of interest. |
| Gene list CSV | The final gene list and the number of probe sets for each gene. |
| Panel design summary HTML | See guidance on interpreting the panel design summary. |
| JSON file | A file describing the custom panel that is used by the Xenium Analyzer instrument (same format as gene_panel.json). |
| Probe details CSV file | This file contains a summary of predicted off-target binding activity for each probe on your panel, based on sequence similarity. |
| RNA-binding domain sequences CSV | If submitted to the Xenium Panel Designer, a file that contains the RNA targets and RNA sequences of the probes (binder) for your advanced customization panel design. |
Find all Xenium In Situ support resources for preparing tissues, running the instrument, and analyzing data using your custom panel here:
If you would like to know the status of your order, email 10x customer service at customerservice@10xgenomics.com with the panel design ID, as well as the sales order and/or PO number.
For custom panel designs, after shipment has been initiated, your order will include a gene panel file (JSON format). You can download the finalized panel design JSON file from the Xenium Panel Designer.
The JSON file should be uploaded to the Xenium Analyzer instrument by inserting a USB into the Xenium Analysis Computer USB port (exFAT format). The Xenium Analyzer User Guide details the steps to start an instrument run.