Peripheral blood mononuclear cells (PBMCs) from a healthy donor - Manual (channel 1)
Single Cell Gene Expression dataset analyzed using Cell Ranger 3.1.0
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
PBMCs isolated and cryopreserved by AllCells. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
Libraries were prepared following the Chromium Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevD). 8 libraries were prepared on 1 chip. This is channel 1 of the chip.
- 5,000 cells targeted
- 4,339 cells detected
- Sequenced on Illumina NovaSeq with approximately 52,296 reads per cell
- 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
- run with --expect-cells=5000
This dataset is licensed under the Creative Commons Attribution license.