Peripheral blood mononuclear cells (PBMCs) from a healthy donor - Manual (channel 1)
Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.1.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
PBMCs isolated and cryopreserved by AllCells. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
Libraries were prepared following the Chromium Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevD). 8 libraries were prepared on 1 chip. This is channel 1 of the chip.
- 5,000 cells targeted
- 4,339 cells detected
- Sequenced on Illumina NovaSeq with approximately 52,296 reads per cell
- 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
- run with --expect-cells=5000
This dataset is licensed under the Creative Commons Attribution license.