20k Human PBMCs, 3' HT v3.1, Chromium X
Single Cell Gene Expression dataset analyzed using Cell Ranger 6.1.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25-30 were obtained by 10x Genomics from AllCells.
Libraries were generated from ~33,000 cells (23,837 cells recovered) as described in the Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 User Guide (CG000416 Rev A) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to a read depth of appriximately 35,000 mean reads per cell.
Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).
Run with --expect-cells=20000.
This dataset is licensed under the Creative Commons Attribution license.