20k Human PBMCs, 3' HT v3.1, Chromium X

Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25-30 were obtained by 10x Genomics from AllCells.

Libraries were generated from ~33,000 cells (23,837 cells recovered) as described in the Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 User Guide (CG000416 Rev A) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to a read depth of appriximately 35,000 mean reads per cell.

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=20000.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.