20k Human PBMCs, 3' HT v3.1, Chromium X
Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.1.0
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25-30 were obtained by 10x Genomics from AllCells.
Libraries were generated from ~33,000 cells (23,837 cells recovered) as described in the Chromium Next GEM Single Cell 3' HT Reagent Kits v3.1 User Guide (CG000416 Rev A) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to a read depth of appriximately 35,000 mean reads per cell.
Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).
Run with --expect-cells=20000.
This dataset is licensed under the Creative Commons Attribution license.