T cells from BALB/c Mice 1k cells - multi (v2)

Universal 5' Gene Expression dataset analyzed using Cell Ranger 5.0.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

BALB/c mouse PBMCs cells were obtained by 10x Genomics from C&M Lab Pro and CD3+ T cells were isolated by immunomagnetic selection.

Gene Expression and TCR Enriched libraries were generated from 1,640 cells (62% viable, expected cell recovery 610) as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A) and sequenced on an Illumina NovaSeq 6000.

These libraries were analyzed using the new Cell Ranger multi pipeline, which combines Gene Expression, Feature Barcode, TCR enriched, and BCR enriched libraries from the same sample. Cell Ranger multi enables consistent cell calling between gene expression and VDJ libraries.

Sequencing Configuration

Paired-end (26X90), dual-indexed sequencing. Read 1: 26 cycles, i7 index: 10 cycles, i5 index: 10 cycles, Read 2: 90 cycles

This dataset is licensed under the Creative Commons Attribution license.