NSCLC tumor - Ig enrichment from amplified cDNA
Universal 5' Gene Expression dataset analyzed using Cell Ranger 2.2.0
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Dataset overview
Output and supplemental files
Input files
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Targeted enrichment of B cell Igs from a fresh surgical resection of a squamous non-small cell lung carcinoma tumor.
The same cDNA was used to generate gene expression (vdj_v1_hs_nsclc_5gex) and TCR (vdj_v1_hs_nsclc_t) specific libraries.
Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000086 Rev C).
- ~15,000 cells loaded
- 2,927 expected Ig-expressing cells recovered (based on CD79A/CD79B expressing fraction in the gene expression data)
- 2,993 cells detected with either single IgH or IgL/IgK or both
- 1,560 cells with productive V-J spanning (IgH, IgL/IgK) pair
- Sequenced on an Illumina NovaSeq 6000 with NovaSeq software v1.2 with ~14,000 read pairs per cell
- 150bp read1 (including 16bp Chromium barcode, 10bp UMI, 13bp switch oligo and transcript), 150bp read2 (transcript), and 8bp i7 sample barcode read configuration
This dataset is licensed under the Creative Commons Attribution license.