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Mouse Splenocytes: 5’v2 Whole Transcriptome Analysis

Universal 5' Gene Expression dataset analyzed using Cell Ranger 6.0.1

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C57Bl/6 mouse splenocytes were obtained by 10x Genomics from C&M Lab Pro. Whole transcriptome/Gene Expression libraries were generated as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A). This whole transcriptome library was used as the parent library for downstream target enrichment. Data were analyzed with Cell Ranger 6.0.1.

  • Sequencing instrument: Illumina NovaSeq 6000
  • Sequencing depth: 46,637 mean reads per cell
  • Sequencing configuration: Paired-end (28 X 90), single-indexed. Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
  • Cells detected: 1,358
  • Median genes per cell: 1,844
  • Median UMIs per cell: 4,372

This dataset is licensed under the Creative Commons Attribution license.