5k Human Melanoma Tumor Isolated with Chromium Nuclei Isolation Kit
Universal 5' Gene Expression dataset analyzed using Cell Ranger 7.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Nuclei were isolated from ~25 mg of fresh frozen human melanoma purchased from Discovery Life Sciences following the Chromium Nuclei Isolation Reagent Kits Sample Prep User Guide (CG000505).
Gene Expression libraries were prepared following the Chromium Single Cell 5'v2 Reagent Kits User Guide (CG000331) and targeting 5,000 cells. Libraries were sequenced on an Illumina NovaSeq instrument targeting 20,000 reads per cell.
Sequencing data were processed using Cell Ranger, with web_summary.html and output files available in relevant tabs.
- Sequencing instrument: Illumina NovaSeq 6000
- Sequencing depth: 86,324 mean reads per cell
- Sequencing configuration: Paired-end, dual indexing;
- Read 1: 28 cycles (16 bp barcode, 12 bp UMI)
- i5 index: 10 cycles (sample index)
- i7 index: 10 cycles (sample index)
- Read 2: 90 cycles (transcript)
- Cells detected: 5,700
- Median genes per cell: 1,220
- Median UMIs per cell: 1,885
This dataset is licensed under the Creative Commons Attribution license.