5k 1:1 Mixture of Human HEK293T and Mouse NIH3T3 Cells, Chromium NextGEM Single Cell 5'
Universal 5' Gene Expression dataset analyzed using Cell Ranger 8.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
A 1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells, obtained by 10x Genomics from ATCC, was used in a classic experiment to demonstrate single cell behavior.
Gene Expression libraries were generated as described in the Chromium Single Cell 5' Reagent Kits User Guide (v2 - Dual Index) User Guide (CG000331) and sequenced on an Illumina NovaSeq 6000 with approximately 50,000 read pairs per cell.
Paired-end, dual indexing:
- 28 cycles Read 1
- 10 cycles i7
- 10 cycles i5
- 90 cycles Read 2
The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.
This dataset is licensed under the Creative Commons Attribution license.