5k Peripheral Blood Mononuclear Cells (PBMCs) from a Healthy Donor(Next GEM v1.1)

Epi ATAC dataset analyzed using Cell Ranger ATAC 1.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Peripheral blood mononuclear cells (PBMCs) from a healthy donor.

Libraries were prepared following the Chromium Next GEM Single Cell ATAC Reagent Kits v1.1 (User Guide CG000209 Rev A). All PBMC libraries (v1.1 chemistry vs. v1 chemistry at all cell loads) were prepared with the same nuclei preparation.

~7,700 transposed nuclei were loaded. ~5,000 expected nuclei recovered. 5,234 nuclei were detected
Sequenced on Illumina NovaSeq with approximately 41k read pairs per cell
- 50 bp Read 1
- 8 bp i7 index (sample index)
- 16 bp i5 (10x Barcode)
- 49 bp Read 2

This dataset is licensed under the Creative Commons Attribution license.