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5k Cells from a combined cortex, hippocampus and subventricular zone of an E18 mouse (Next GEM)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.0.2

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Cells from a combined cortex, hippocampus and sub ventricular zone of an E18 mouse (the same cells were used to generate 5k_neuron_v3).

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevA).

  • 6,566 cells detected
  • Sequenced on Illumina NovaSeq with approximately 78,597 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.