20k Human PBMCs Multiplex Sample (Donors 1-4)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 9.0.0

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Dataset overview

Output and supplemental files

Input files

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Peripheral blood mononuclear cells (PBMCs) from two healthy human male donors, aged 18-35 and two healthy female donors, aged 36-50 were obtained by 10x Genomics from Cellular Technologies Limited.

Gene Expression libraries were generated as described in the GEM-X Universal 3' Gene Expression v4 4-plex On-chip Multiplexing User Guide (CG000768) and sequenced on an Illumina NovaSeq 6000 with approximately 53,000 read pairs per cell for the Gene Expression library.

Paired-end, dual indexing libraries were sequenced with this configuration:

28 cycles Read 1
10 cycles i7
10 cycles i5
90 cycles Read 2

Libraries were analyzed using the cellranger multi pipeline.

See companion datasets:

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.