10k Mouse E18 Combined Cortex, Hippocampus and Subventricular Zone Cells, Chromium NextGEM Single Cell 3'

Universal 3' Gene Expression dataset analyzed using Cell Ranger 8.0.0

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Dataset overview

Output and supplemental files

Input files

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Cells from a combined cortex, hippocampus, and subventricular zone of an E18 mouse tissue (C57BL/6 strain) were obtained by 10x Genomics from BrainBits LLC. Brain tissue was dissociated following the 10x Demonstrated Protocol for Dissociation of Mouse Embryonic Neural Tissue for Single Cell RNA Sequencing (CG00055).

Gene Expression libraries were generated as described in the Chromium Single Cell 3' Reagent Kits User Guide (v3.1 - Dual Index) User Guide (CG000315) and sequenced on an Illumina NovaSeq 6000 with approximately 80,000 read pairs per cell.

Paired-end, dual indexing:

28 cycles Read 1
10 cycles i7
10 cycles i5
90 cycles Read 2

The cellranger multi pipeline in Cell Ranger v8.0.0 was used to analyze libraries.

This dataset is licensed under the Creative Commons Attribution license.