10k Human PBMCs, ATAC v1.1, Chromium Controller
Epi ATAC dataset analyzed using Cell Ranger ATAC 2.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human peripheral blood mononuclear cells (PBMCs) of a healthy male donor aged 30-35 were obtained by 10x Genomics from AllCells. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell ATAC Sequencing (CG000169).
ATAC libraries were generated from ~15,400 transposed nuclei (8,352 nuclei recovered) as described in the Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry) (CG000209 Rev E) using the Chromium Controller and sequenced on an Illumina NovaSeq 6000 to approximately 60k read pairs per cell.
50 cycles Read 1, 8 cycles i7 (sample index), 16 cycles i5 (10x barcode), 49 cycles Read 2.
This dataset is licensed under the Creative Commons Attribution license.