1k 1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells (Next GEM)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.0.2

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Dataset overview

Output and supplemental files

Input files

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1:1 mixture of fresh frozen human (HEK293T) and mouse (NIH3T3) cells.

This is a classic human-mouse mixture experiment to demonstrate single cell behavior (the same cells were used to generate 1k_hgmm_v3, 5k_hgmm_v3_nextgem, 10k_hgmm_v3_nextgem)).

Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide (CG000204 RevA).

1,230 cells detected
Sequenced on Illumina NovaSeq with approximately 66,606 reads per cell
28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
run with --expect-cells=1000

This dataset is licensed under the Creative Commons Attribution license.