The web summary file (web_summary. html) output by the cellranger multi pipeline is the initial point of reference for determining sample performance in the Chromium Fixed RNA Profiling (Single Cell Gene Expression Flex) assay. This Technical Note presents an overview of web summary file interpretation, including the expected metrics and characteristic plots for Gene Expression libraries generated using this Fixed RNA Profiling (FRP) assay. Metrics associated with product extensions (i.e., Feature Barcode technology) are not included in this document.