Plan ahead by referring to the list of equipment, kits, and reagents needed for Flex Gene Expression.

Experimental Design

Experimental Design & Planning

### What is Fixed RNA Profiling with Protein Labeling?

* Reveal gene expression and cell surface protein from the same fixed cell
* [To learn more click here](/products/flex-gene-expression)
* Compatible with Singleplex or Multiplex Samples via Direct Capture (TotalSeq™️-B) or Barcode Oligo Capture (TotalSeq™️-C)

### Fixed RNA Profiling Feature Barcode Compatibility Table

| Version  | Plexing    | Direct Capture | Barcode Oligo Capture | Compatible Feature Barcode Kit                          | Antibody Compatibility |
| -------- | ---------- | -------------- | --------------------- | ------------------------------------------------------- | ---------------------- |
| Next GEM | Singleplex | <Check />      |                       | Fixed RNA Feature Barcode Kit (PN-1000419)              | TotalSeq™️-B           |
| Next GEM | Multiplex  |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |
| GEM-X    | Singleplex |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |
| GEM-X    | Multiplex  |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |

Reveal gene expression and protein expression from the same fixed cell.

Fixed RNA Profiling with Cell Surface Protein Labeling

Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Additional Applications

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

<Text as={"p"} size={"small"} style={{ lineHeight: "28px"}} weight={"regular"}>To check if a Demonstrated Protocol is compatible with a specific version, refer to the <Anchor href={"/support/universal-three-prime-gene-expression/documentation/steps/sample-prep/single-cell-flex-gene-expression-protocol-compatibility-table"}><Text as={"span"} color={"inherit"} size={"small"} weight={"medium"}>{"Demonstrated Protocol Compatibility Table"}</Text></Anchor><Text as={"span"} size={"small"} weight={"regular"}>.</Text></Text>

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Flex Gene Expression assay.

Probe Sets

Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.