Construct a sequencing-ready single cell library by following our step-by-step User Guide / Protocol.

Library Construction

### What is Fixed RNA Profiling with Protein Labeling?

* Reveal gene expression and cell surface protein from the same fixed cell
* [To learn more click here](/products/flex-gene-expression)
* Compatible with Singleplex or Multiplex Samples via Direct Capture (TotalSeq™️-B) or Barcode Oligo Capture (TotalSeq™️-C)

### Fixed RNA Profiling Feature Barcode Compatibility Table

| Version  | Plexing    | Direct Capture | Barcode Oligo Capture | Compatible Feature Barcode Kit                          | Antibody Compatibility |
| -------- | ---------- | -------------- | --------------------- | ------------------------------------------------------- | ---------------------- |
| Next GEM | Singleplex | <Check />      |                       | Fixed RNA Feature Barcode Kit (PN-1000419)              | TotalSeq™️-B           |
| Next GEM | Multiplex  |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |
| GEM-X    | Singleplex |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |
| GEM-X    | Multiplex  |                | <Check />             | Fixed RNA Feature Barcode Multiplexing Kit (PN-1000628) | TotalSeq™️-C           |

Reveal gene expression and protein expression from the same fixed cell.

Fixed RNA Profiling with Cell Surface Protein Labeling

Protein

It is critical to choose the User Guide / Protocol that corresponds to your Reagent Kit version. Find the right User Guide via the button below.

Additional Applications

Plan ahead by referring to the list of equipment, kits, and reagents needed for Flex Gene Expression.

Experimental Design

Experimental Design & Planning

Prepare single cell/nuclei suspension from fresh samples for fixation or from archived samples by following our Demonstrated Protocols.

Sample Prep

<Text as={"p"} size={"small"} style={{ lineHeight: "28px"}} weight={"regular"}>To check if a Demonstrated Protocol is compatible with a specific version, refer to the <Anchor href={"/support/universal-three-prime-gene-expression/documentation/steps/sample-prep/single-cell-flex-gene-expression-protocol-compatibility-table"}><Text as={"span"} color={"inherit"} size={"small"} weight={"medium"}>{"Demonstrated Protocol Compatibility Table"}</Text></Anchor><Text as={"span"} size={"small"} weight={"regular"}>.</Text></Text>

Find a list of probes targeting protein-coding genes in human and mouse transcriptomes for the Flex Gene Expression assay.