Chromium Single Cell
Universal 5' Gene Expression
RT-based assays using GEM-X techology to analyze immune diversity
Up to 80% cell recovery
>40% higher gene sensitivity vs other methods
More usable reads, save up to 50% on sequencing costs
Why choose Universal 5' Gene Expression
Assay specifications
Species
Mouse
Human
Rat (except with TCR/BCR)
Non-human primate (except with TCR/BCR)
and moreCompatible samples
Cell suspension
Nuclei suspension
Fresh tissue
Frozen tissue
Flow-sorted cells
Fixed human PBMCs
OrganoidsMultiomics
5' gene expression
Cell surface protein
TCR/BCR sequencing
CRISPR screening
Antigen specificityCompatible instruments
Throughput options
Learn moreSingleplex
20K cells/sample
160K cells/chip
8 samples/chipComponents
Each experiment requires a chip, index kit, and core reagents plus an optional feature barcoding kit. If you're unsure what you need, use the experiment builder find the custom list of items required for your experiment.
Consumables
Choose a version
Nuclei Isolation (optional)
1000494
Chromium Nuclei Isolation with RNase Inhibitor Kit, 16rxns
Chip
1000698
Chromium GEM-X Single Cell 5' Chip Kit v3, 4 chips
Index Kit
1000215
Dual Index Kit TT Set A 96 rxns
1000250
Dual Index Kit TN Set A, 96 rxn
Core Reagents
1000695
Chromium GEM-X Single Cell 5' Kit v3, 4 rxns
1000699
Chromium GEM-X Single Cell 5' Kit v3, 16 rxns
V(D)J Amplification Kit (optional)
1000252
Chromium Single Cell Human TCR Amplification Kit, 16 rxns
1000253
Chromium Single Cell Human BCR Amplification Kit, 16 rxns
1000254
Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns
1000255
Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns
1000694
Library Construction Kit C, 16 rxns
CRISPR Kit (optional)
1000451
5' CRISPR Kit, 16 rxns
Feature Barcoding Kit (optional)
1000703
Chromium GEM-X Single Cell 5 Feature Barcode Kit v3, 16 rxns
Build your own Universal 5' Gene Expression experiment
Design the experiment that's right for you
Documents and resources
Product sheet
User guides
Sequencing requirements
Minimum 20,000 read pairs/cell for gene expression; 5,000 read pairs/cell for V(D)J, protein, CRISPR analysis.
View allVideos
Chromium GEM-X intro webinar: See the next generation of single cell in action
Data analysis
Cell Ranger
Cell Ranger is a set of free analysis pipelines for processing Chromium Single Cell data
Learn more
Loupe Browser
Loupe Browser is a desktop application for Windows and MacOS that allows you to interactively visualize data
Learn more
Cloud Analysis
Cloud Analysis enables quick and easy access to Cell Ranger pipelines from your web browser
Learn moreFeatured publications
Combined PD-1, BRAF and MEK inhibition in BRAFV600E colorectal cancer: a phase 2 trial
Nature Medicine, 2023
Jun Tian, et al.
Genomic and immune signatures predict clinical outcome in newly diagnosed multiple myeloma treated with immunotherapy regimens
Nature Cancer, 2023
Francesco Maura, et al.
Transcriptional signatures associated with persisting CD19 CAR-T cells in children with leukemia
Nature Medicine, 2023
Nathaniel Anderson, et al.
Featured datasets
10k Human DTC Melanoma, Chromium GEM-X Single Cell 5'
View dataset10k Human PBMCs Stained with TotalSeq™-C Human TBNK Cocktail, Chromium GEM-X Single Cell 5'
View dataset10k Mouse Splenocytes, Chromium GEM-X Single Cell 5'
View datasetRelated products
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Epi Multiome ATAC + Gene Expression
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Epi ATAC
Single cell profiling of open chromatin