Spatial Gene Expression for Fresh Frozen
What is Spatial Gene Expression for Fresh Frozen?
Visium Spatial Gene Expression measures the whole transcriptome of intact fresh frozen sections in a spatially resolved manner by mapping gene expression over a high-resolution microscope image of the H&E- or IF-stained tissue.
Documentation
Find resources, from protocols to free-to-use analysis tools.
Plan ahead by referring to the list of equipment, kits, and reagents needed for the Visium Spatial protocol.
For proper tissue handling and preparation techniques to preserve the morphology, quality, and integrity of tissue sections, refer to our Tissue Preparation Guide.
Methanol fix, stain, and image tissue following our Demonstrated Protocol.
Optimize permeabilization conditions for your fresh frozen tissue sections by following our step-by step User Guide.
For imaging hardware, general image acquisition and analysis guidelines, and example images, refer to our Technical Note.
Construct a sequencing-ready Visium spatial library from your fresh frozen tissue sections following our step-by-step User Guide.
Use pre-designed panels for targeted gene expression, followed by sequencing and data analysis.
Sequence your Visium spatial library as per our provided recommendations.
Analyze your Visium spatial data using our pipelines and visualization tools.
Datasets
Access free, publicly available data from a number of different sample types generated with Visium spatial solutions
Videos
Explore how-to videos and training modules.
Q&A
Find answers to common technical questions, from sample prep through data analysis, about Visium spatial workflows.
Publications
Read key peer-reviewed research publications offering greater insights into biology, enabled by 10x Genomics products and technologies.
