Peripheral blood mononuclear cells (PBMCs) from a healthy donor - Chromium Connect (channel 5)

Single Cell Gene Expression dataset analyzed using Cell Ranger 3.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

PBMCs isolated and cryopreserved by AllCells. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

Libraries were prepared following the Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits v3.1 (CG000286 RevA) and Software version Assay Build 308. 8 libraries were prepared on 1 chip in 1 run. This is channel 5.

5,000 cells targeted
3,363 cells detected
Sequenced on Illumina NovaSeq with approximately 61,935 reads per cell
28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.