PBMC from a Healthy Donor - Granulocytes Removed Through Cell Sorting (3k)

Cryopreserved human peripheral blood mononuclear cells (PBMCs) from a healthy female donor aged 25 were obtained by 10x Genomics from AllCells.

Granulocytes were removed by cell sorting, followed by nuclei isolation as described in the Demonstrated Protocol- Nuclei Isolation for Single Cell Multiome ATAC + Gene Expression Sequencing (CG000365 Rev A).

Paired ATAC and Gene Expression libraries were generated from the isolated nuclei as described in the Chromium Next GEM Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A) and sequenced on Illumina Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow).

User Guide: Chromium Next Gem Single Cell Multiome ATAC + Gene Expression User Guide (CG000338 Rev A)

Libraries generated:

• Chromium Single Cell Multiome ATAC library
• Chromium Single Cell Multiome Gene Expression library

Sequencing information:

• Sequencer: Novaseq 6000 v1 Kit (Forward Strand Dual-Index Workflow)
• Cycle numbers:
  • Gene Expression library: 28,10,10,90
  • ATAC library: 50,8,16,49

Key metrics:

• Estimated number of cells: 2,711
• ATAC Mean raw read pairs per cell: 28,482
• ATAC Median high-quality fragments per cell: 14,479
• ATAC Number of peaks: 98,319
• GEX Mean raw reads per cell: 60,701
• GEX Median genes per cell: 1,791
• GEX Median UMI counts per cell: 3,790
• Linked genes: 8,664
• Linked peaks: 53,127

This dataset is licensed under the Creative Commons Attribution license.