7.5k Sorted Cells from Human Invasive Ductal Carcinoma, 3’ v3.1
Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Invasive Ductal Carcinoma cells from a female donor aged 65 were obtained by 10x Genomics from Discovery Life Sciences.
Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 (Dual Index) User Guide (CG000315) and sequenced on the Illumina NovaSeq 6000.
Sequencing Depth: ~54,504 mean reads per cell
Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)
Analysis Parameters Used: Run with --expect-cells=7500
This dataset is licensed under the Creative Commons Attribution license.