500 Human PBMCs, 3' LT v3.1, Chromium Controller

Universal 3' Gene Expression dataset analyzed using Cell Ranger 6.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Human peripheral blood mononuclear cells (PBMCs) of a healthy female donor aged 25-30 were obtained by 10x Genomics from AllCells.

Libraries were generated from ~1675 cells (~500 cells recovered) as described in the Chromium Next GEM Single Cell 3' LT Reagent Kits (v3.1 Dual Index) User Guide (CG000399 RevB) using the Chromium Controller and sequenced on an Illumina NovaSeq 6000 to a read depth of 50k mean reads per cell.

Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript).

Run with --expect-cells=500.

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.