5k Peripheral Blood Mononuclear Cells (PBMCs) from a Healthy Donor with a Panel of TotalSeq™-B Antibodies (v3 chemistry)

Universal 3' Gene Expression dataset analyzed using Cell Ranger 3.1.0

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Dataset overview

Output and supplemental files

Input files

Learn about Chromium analysis

Peripheral blood mononuclear cells (PBMCs) from a healthy donor were stained with a panel of 31 TotalSeq™-B antibodies (BioLegend).

PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

Libraries were prepared following the Chromium Single Cell 3ʹ Reagent Kits v3 with Feature Barcoding technology for Cell Surface Protein User Guide (CG000185 RevB).

5,247 cells detected
Sequenced on Illumina NovaSeq with approximately 28,918 reads per cell
- 28 bp Read 1 (16 bp Chromium barcode and 12 bp UMI)
- 91 bp Read 2 (transcript)
- 8 bp i7 (sample barcode)
run with --expect-cells=5000

This dataset is licensed under the Creative Commons Attribution license.