200 Sorted Cells from Human Glioblastoma Multiforme, 3’ LT v3.1
Single Cell Gene Expression dataset analyzed using Cell Ranger 6.0.0
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Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Human Glioblastoma Multiforme cells from a male donor aged 71 were obtained by 10x Genomics from Discovery Life Sciences.
Libraries were generated following the Chromium Next GEM Single Cell 3' LT Reagent Kits v3.1 (Dual Index) User Guide (CG000399) and sequenced on Illumina NovaSeq 6000.
Sequencing Depth: 54,761 mean reads per cell
Paired-end, dual indexing Read 1: 28 cycles (16 bp barcode, 12 bp UMI) i5 index: 10 cycles (sample index) i7 index: 10 cycles (sample index) Read 2: 90 cycles (transcript)
Analysis Parameters Used: Run with --expect-cells=200
This dataset is licensed under the Creative Commons Attribution license.