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10k PBMCs from a Healthy Donor - Gene Expression with a Panel of TotalSeq™-B Antibodies

Single Cell Gene Expression dataset analyzed using Cell Ranger 3.0.0

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Peripheral blood mononuclear cells (PBMCs) from a healthy donor stained with TotalSeq™-B antibodies. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • 7,865 cells detected
  • Sequenced on Illumina NovaSeq with approximately 35,000 reads per cell
  • 28bp read1 (16bp Chromium barcode and 12bp UMI), 91bp read2 (transcript), and 8bp I7 sample barcode
  • run with --expect-cells=10000
  • This dataset was generated using a pre-release set of TotalSeq-B antibodies. The barcode sequences have since changed. Please refer to https://www.biolegend.com/totalseq for the latest conjugated barcode information.

This dataset is licensed under the Creative Commons Attribution license.