10k Human PBMCs, ATAC v1.1, Chromium X
Epi ATAC dataset analyzed using Cell Ranger ATAC 2.0.0
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Dataset overview
Output and supplemental files
Input files
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Human peripheral blood mononuclear cells (PBMCs) of a healthy male donor aged 30-35 were obtained by 10x Genomics from AllCells. Nuclei were isolated using the demonstrated protocol Nuclei Isolation for Single Cell ATAC Sequencing (CG000169).
ATAC libraries were generated from ~15,400 transposed nuclei (8,985 nuclei recovered) as described in the Chromium Single Cell ATAC Reagent Kits User Guide (v1.1 Chemistry) (CG000209 Rev E) using the Chromium X and sequenced on an Illumina NovaSeq 6000 to approximately 50k read pairs per cell.
50 cycles Read 1, 8 cycles i7 (sample index), 16 cycles i5 (10x barcode), 49 cycles Read 2.
This dataset is licensed under the Creative Commons Attribution license.