Chromium Single Cell 5’ Barcode Enabled Antigen Mapping (BEAM) enables multiplexed screening of antigen targets to match unique antigens with their corresponding B-cell receptors (BCRs) and T-cell receptors (TCRs), allowing rapid discovery of antigen-specific BCR (BEAM-Ab) and TCR (BEAM-T), respectively. This is accomplished by assembling antigens or peptides of interest with uniquely barcoded 10x Genomics BEAM Conjugates (16 unique BEAM Conjugates) and by labeling samples with the assembled reagents (BEAM-Ab or BEAM-T Assembly), followed by flow sorting of antigen-specific cells. The cells can then be used with Chromium Single Cell 5' Reagent Kits with Feature Barcode technology for Barcode Enabled Antigen Mapping (BEAM) to generate BEAM, 5' Gene Expression, and V(D)J libraries. This document provides an overview of the key considerations for the various steps of the workflow when planning a BEAM experiment.
