Protocols labeled "2.0" are compatible with Visium HD 6.5 mm and 11 mm slides and contain a "Featured" tag. For ongoing Visium HD 6.5 mm projects, please use original protocol versions.
When working with Fixed Frozen tissues, generating sections with uniform slide adhesion can be challenging due to their sample composition. Please refer to the Visium HD Spatial Gene Expression Protocol Planner (CG000698) Tested Slides section for slide part number recommendations and additional considerations.
This document provides guidance on preparation of Fixed Frozen tissue blocks and generating tissue slides. Demonstrated protocols for performing tissue staining (H&E and IF) are included.
Key companion documents for use with this document:
- Visium HD Spatial Gene Expression Protocol Planner (CG000698)
- Tissue Slide Alignment Instruction, Quick Reference Cards (CG000548)
- Visium S3 for Tissue Slide Cassette Assembly Quick Reference Cards (CG000730)
For use with:
- Visium HD, Human Transcriptome, 6.5 mm, PN-1000675 (4 rxns) / PN-1000673 (16 rxns)
- Visium HD, Mouse Transcriptome, 6.5 mm, PN -1000676 (4 rxns) / PN-1000674 (16 rxns)
- Visium CytAssist Reagent Accessory Kit, PN-1000499
- Dual Index Kit TS Set A, 96 rxns, PN-1000251