The tissue types listed below were tested in-house using:
Next GEM Flex Gene Expression (Fixed RNA Profiling)
- Tissue Fixation & Dissociation for Chromium Fixed RNA Profiling Demonstrated Protocol (CG000553) or
- Chromium Nuclei Isolation Kit (CG000505) followed by the Fixation of Cells & Nuclei for Chromium Fixed RNA Profiling (CG000478)
GEM-X Flex Gene Expression
- Tissue Fixation & Dissociation for GEM-X Flex Gene Expression (CG000783) or
- Chromium Nuclei Isolation Kit (CG000505) followed by the Fixation of Cells & Nuclei for GEM-X Flex Gene Expression (CG000782)
Listed below are the cell yields from 25 mg of various mouse and human tissue types (flash-frozen or fresh). Variability of performance may be observed based on sample handling, quality, composition, and other properties.
If in the middle of Chop/Fix experiments, we recommend keeping the enzymes consistent.
Mouse (Healthy) Tested Tissue List
- List of mouse tissue types that were tested in-house for compatibility with Flex.
| Tissue | Tissue Fixation & Dissociation* (Flash-frozen tissue) | Tissue Fixation & Dissociation* (Fresh tissue) | Nuclei Isolation** (Flash-frozen tissue) | Nuclei Isolation** (Fresh tissue) | Additional Notes |
|---|---|---|---|---|---|
| Adipose | Not recommended | Not recommended | 3.00E+04 | Not tested | High lipid content in adipose cells prevents cells from pelleting (cells are buoyant). Nuclei isolation recommended |
| Bladder | 7.00E+05 | Not tested | 1.70E+05 | Not tested | |
| Brain (Forebrain) | 1.00E+06 | 7.90E+05 | 7.10E+05 | 3.00E+05 | |
| Brain (Cerebellum) | Not tested | 3.10E+06 | Not tested | 5.20E+05 | |
| Brain (Whole) | 1.60E+06 | 8.40E+05 | 7.90E+05 | 7.00E+05 | |
| Colon | 2.20E+06 | Not tested | 9.80E+05 | Not tested | |
| Eye | 1.60E+06 | Not tested | 3.70E+05 | Not tested | |
| Heart | 9.90E+05 | 2.00E+06 | 3.00E+04 | Not tested | Low yield; Tissue Fixation & Dissociation recommended |
| Intestine | 3.10E+06 | 1.60E+06 | 9.00E+05 | 2.50E+04 | |
| Kidney | 2.70E+06 | 2.50E+06 | 1.20E+06 | 1.00E+06 | |
| Liver | 1.30E+06 | 5.70E+05 | 1.00E+06 | 2.80E+05 | |
| Lung | 2.00E+06 | 3.10E+06 | 7.40E+05 | 3.40E+05 | |
| Muscle | 4.10E+05 | Not tested | 1.10E+04 | Not tested | Lower than expected yield using Nuclei Isolation Kit. Tissue Fixation & Dissociation protocol is recommended to improve cell yield. |
| Ovary | 3.20E+06 | Not tested | 1.20E+06 | Not tested | |
| Pancreas | 1.80E+06 | 9.20E+05 | 2.70E+05 | 1.90E+04 | Tissue Dissociation & Fixation recommended; Digestion using Liberase TH 1 mg/mL recommended. No greater than 100k cells into Probe Hybridization for optimal performance. |
| Spinal Cord | 1.50E+06 | Not tested | 2.20E+05 | Not tested | High debris |
| Spleen | 1.40E+07 | 6.30E+06 | 1.20E+05 | 5.30E+05 | Nuclei prone to clumping, Tissue Dissociation & Fixation recommended. No greater than 100k cells into Probe Hybridization for optimal performance. |
| Stomach | 2.50E+06 | Not tested | 3.60E+05 | Not tested | |
| Testis | 4.20E+06 | Not tested | 8.60E+05 | Not tested | |
| Thymus | 1.50E+07 | 2.60E+07 | 4.30E+04 | 7.90E+06 | Nuclei are prone to clumping. Tissue Fixation & Dissociation protocol recommended. |
| Tongue | 1.40E+06 | Not tested | 2.10E+04 | Not tested |
Human Tested Tissue List
* List of human tissue types that were tested in-house and found to perform well with Flex.
| Tissue | Tissue Fixation & Dissociation* (Flash-frozen tissue) | Tissue Fixation & Dissociation* (Fresh tissue) | Nuclei Isolation** (Flash-frozen tissue) | Nuclei Isolation** (Fresh tissue) | Additional Notes |
|---|---|---|---|---|---|
| Jejunum | 1.37E+06 | Not tested | 4.18E+05 | Not tested | |
| Colon | 4.00E+04 | Not tested | 6.10E+05 | Not tested | Lower than expected yield from 25 mg colon tissue; using >25 mg tissue recommended |
| Lung | 3.40E+06 | Not tested | Not tested | Not tested | |
| Kidney | 2.00E+06 | Not tested | Not tested | Not tested | |
| Liver | 1.80E+06 | Not tested | 6.60E+05 | Not tested | |
| Spleen | 9.60E+06 | Not tested | 6.50E+06 | Not tested | |
| Ileum | 1.40E+05 | Not tested | 2.37E+05 | Not tested | |
| Testes | 2.05E+06 | Not tested | 8.08E+05 | Not tested | |
| Duodenum | 3.20E+05 | Not tested | 1.28E+05 | Not tested | |
| Breast Cancer | 9.60E+05 | Not tested | 9.20E+05 | Not tested | |
| Melanoma | 3.18E+06 | Not tested | 5.98E+05 | Not tested | |
| Ovarian Cancer | 9.30E+05 | Not tested | 6.93E+05 | Not tested | |
| Pancreatic Cancer | 1.78E+06 | Not tested | 9.11E+05 | Not tested | |
| Tonsils | 1.76E+07 | Not tested | 5.15E+05 | Not tested |
* Tissue Fixation & Dissociation (CG000553)
** Nuclei Isolation (Chromium Nuclei Isolation Kit, CG000505) followed by Sample fixation (CG000478)