The Visium Spatial Gene Expression for FFPE Reagent Kits workflow produces Visium Spatial Gene Expression sequencing-ready libraries for measuring RNA in intact formalin fixed paraffin embedded (FFPE) tissue sections. This Technical Note presents a comparison of sequencing metrics for pooled Visium Spatial Gene Expression for FFPE libraries across Illumina️ platforms. The expected base percentage profiles and Phred quality scores (Q scores) based on a control library are described to provide general guidance on the expected range of sequencing metrics on Illumina platforms. Individual results may vary depending on the specific sequencing instrument and/or particular sample and loading characteristics.
