The Chromium Next GEM Single Cell 5' v2 (dual index), standard, and High Throughput (HT) workflow produces sequencing-ready Gene Expression, V(D)J, Cell Surface Protein, CRISPR, and/or Barcode Enabled Antigen Mapping (BEAM) libraries from the same single cells. This enables simultaneous profiling of cellular features along with gene expression profiling. This Technical Note presents a comparison of sequencing metrics for various Single Cell 5' v2 Dual Index library types across Illumina platforms. Results may vary depending on the sequencing instrument and/or sample and loading characteristics.

FOR USE WITH:

• Chromium Next GEM Single Cell 5' Kit v2, 16 rxns PN-1000263
• Chromium Next GEM Single Cell 5' Kit v2, 4 rxns PN-1000265
• Library Construction Kit, 16 rxns PN-1000190
• 5' Feature Barcode Kit, 16 rxns PN-1000256
• Chromium Single Cell Human TCR Amplification Kit, 16 rxns PN-1000252
• Chromium Single Cell Human BCR Amplification Kit, 16 rxns PN-1000253
• Chromium Single Cell Mouse TCR Amplification Kit, 16 rxns PN-1000254
• Chromium Single Cell Mouse BCR Amplification Kit, 16 rxns PN-1000255
• Chromium Next GEM Chip K Single Cell Kit, 48 rxns PN-1000286
• Chromium Next GEM Chip K Single Cell Kit, 16 rxns PN-1000287
• Dual Index Kit TT Set A, 96 rxns PN-1000215
• Dual Index Kit TN Set A, 96 rxns PN-1000250