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Immune Profiling HT Demonstrated Protocol Compatibility Table

Immune Profiling HT Demonstrated Protocol Compatibility Table

ProtocolHTHT with Cell Surface Protein & Immune Receptor Mapping
Single Cell Protocols - Cell Preparation Guide✓✓
Enrichment of CD3+ T Cells from Dissociated Tissues for Single Cell RNA Sequencing and Immune Repertoire Profiling✓✓*
Tumor Dissociation for Single Cell RNA Sequencing✓*✓*
Thawing Dissociated Tumor Cells for Single Cell RNA Sequencing✓✓*
Single Cell Suspensions from Cultured Cell Lines for Single Cell RNA Sequencing✓✓
Removal of Dead Cells from Single Cell Suspensions for Single Cell RNA Sequencing✓✓*
Fresh Frozen Human-Mouse Cell Line Mixtures for Single Cell RNA Sequencing✓✓
Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing✓✓
Cell Surface Protein Labeling for Single Cell RNA Sequencing Protocols✘✓
Cell Labeling with Dextramers for Single Cell RNA Sequencing Protocols*✘✓
Methanol Fixation of Cells for Single Cell RNA Sequencing✘*✘
Isolation of Nuclei for Single Cell RNA Sequencing & Tissues for Single Cell RNA Sequencing✘*✘

✓ Compatible (tested in-house) ✓* Untested but low risk ✘* Untested but high risk ✘ Not compatible *Antigen-only analysis is not currently supported by 10x Genomics. For antigen analysis, we recommend staining with dextramer reagents in conjunction with a small panel of TotalSeq C antibodies. This increases the cell calling accuracy of the data. Please contact Tech Support for TotalSeq C antibody recommendations.

Document Type
Demonstrated Protocol
Last Modified
June 17, 2022