Spatial whole transcriptome profiling of the tumor microenvironment in archived and freshly-mounted FFPE tissues
The constant interaction between a tumor and its microenvironment plays a critical role in how the cancer develops, progresses, and responds to therapies. Traditional tissue-based studies of the TME may limited to a small number of target analytes, which can limit biological insights. Here, we used the Visium CytAssist instrument and the Visium CytAssist Spatial Gene Expression Kit for FFPE to profile the whole transcriptome and immunofluorescence-based protein expression from 11-month old archived and freshly-preserved cancer samples. Our data provide a more comprehensive understanding of cellular behavior in and around tumors, yielding new insights into disease progression, predictive biomarkers, and therapeutic responses.
The constant interaction between a tumor and its microenvironment plays a critical role in how the cancer develops, progresses, and responds to therapies. Traditional tissue-based studies of the TME may limited to a small number of target analytes, which can limit biological insights. Here, we used the Visium CytAssist instrument and the Visium CytAssist Spatial Gene Expression Kit for FFPE to profile the whole transcriptome and immunofluorescence-based protein expression from 11-month old archived and freshly-preserved cancer samples. Our data provide a more comprehensive understanding of cellular behavior in and around tumors, yielding new insights into disease progression, predictive biomarkers, and therapeutic responses.