Human Colorectal Cancer: Whole Transcriptome Analysis
Spatial Gene Expression dataset analyzed using Space Ranger 1.2.0
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10X Genomics obtained fresh frozen Invasive Adenocarcinoma of the large intestine tissue from BioIVT Asterand. The tissue was embedded and cryosectioned as described in Visium Spatial Protocols – Tissue Preparation Guide (Demonstrated Protocol CG000240). Tissue sections of 10µm were placed on Visium Gene Expression slides and fixed and stained following Methanol Fixation, H&E Staining & Imaging for Visium Spatial Protocols (CG000160).
The tissue was AJCC/UICC T4aN0M0, Stage Group IIB.
The H&E image was acquired using a Nikon Eclipse Ti2-E microscope with the following settings:
- Color camera
- 10X objective
- Numerical Aperture: 0.45
- Exposure: 20 ms
The Visium Gene Expression library (T1T2-E8) was prepared as described in the Visium Spatial Reagent Kits User Guide (CG000239 Rev D). Sequencing data was processed using Space Ranger.
- Sequencing instrument: Illumina NovaSeq 6000, flow cell HHYWHDSXY (lanes 1-4)
- Sequencing depth: 112,228 mean reads per cell
- Sequencing configuration: Paired-end (28 X 90), Dual-Indexed Sequencing. Read 1: 28 cycles (16 bp barcode, 12 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).
- Slide: V10A13-206
- Area: C1
Key metrics were:
- Spots detected: 3,138
- Median genes per spot: 3,538
- Median UMI counts per spot: 8,906
To maintain donor anonymity, sequence data for this sample is not currently available.
This dataset is licensed under the Creative Commons Attribution license.