This protocol outlines the steps necessary to reset Visium Spatial slides containing incorrectly placed fresh frozen tissue for reuse with 10x Genomics Visium Spatial protocols. This protocol is compatible with both Visium Spatial Tissue Optimization and Visium Spatial Gene Expression slides. Slides must be reset prior to tissue permeabilization; once permeabilization has been started, slides can no longer be reused.
 | This protocol is only compatible with the Visium assay for Fresh Frozen tissues. Do NOT use with FFPE tissues. |
FOR USE WITH:
- Visium Spatial Tissue Optimization Slide & Reagent Kit, 4 slides PN-1000193
- Visium Spatial Gene Expression Slide & Reagent Kit, 16 rxns PN-1000184
- Visium Spatial Gene Expression Slide & Reagent Kit, 4 rxns PN-1000187
- Visium Gateway Tissue Optimization Slide & Reagent Kits, 1 slide (PN-1000313, PN-1000314)
- Visium Gateway Gene Expression Slide & Reagent Kit, 2 rxns PN-1000317
- Visium Accessory Kit, PN-1000194
- Dual Index Kit TT Set A, 96 rxns PN-1000215