Technical Note - Chromium Single Cell V(D)J Libraries – Sequencing Metrics for Illumina® Sequencers

This Technical Note presents a comparison of Chromium Single Cell V(D)J libraries sequenced across different Illumina platforms and describes the differences seen for several key sequencing metrics. Chromium Single Cell V(D)J libraries can be sequenced using a paired-end configuration with single indexing on several Illumina sequencing platforms. Each platform differs in loading concentration, PhiX spike-in, resulting cluster densities (or %PF), and sequencing quality metrics (Q30) depending on which Illumina instrument was used. This document is intended to give general guidance on the expected range of sequencing metrics on multiple platforms, based on a set of controlled libraries. Individual results may still vary, depending on the specific sequencing instrument and/or particular sample and loading characteristics.

FOR USE WITH

• Chromium™ Single Cell 5’ Library & Gel Bead Kit, 16 rxns PN-1000006
• Chromium™ Single Cell 5’ Library & Gel Bead Kit, 4 rxns PN-1000014
• Chromium™ Single Cell V(D)J Enrichment Kit, Human T Cell, 96 rxns PN-1000005
• Chromium™ Single Cell A Chip Kit, 48 rxns PN-120236
• Chromium™ Single Cell A Chip Kit, 16 rxns PN-1000009
• Chromium™ i7 Multiplex Kit, 96 rxns PN-120262