Chromium Single Cell 5’ Barcode Enabled Antigen Mapping (BEAM) enables multiplexed screening of antigen targets to match unique antigens with their corresponding B-cell receptors (BCRs) and T-cell receptors (TCRs), allowing rapid discovery of antigen-specific BCR (BEAM-Ab) and TCR (BEAM-T), respectively. This is done by assembling antigens/peptides of interest with uniquely barcoded 10x Genomics BEAM Conjugates and by labeling samples with the assembled reagents (BEAM-Ab or BEAM-T Assembly), followed by flow sorting of antigen-specific cells. The cells can then be used with Chromium Single Cell 5' Reagent Kits with Feature Barcode technology for BEAM to generate BEAM, 5' Gene Expression, and V(D)J libraries. This document provides workflow overview and additional equipment, kits, and reagents for the Chromium Single Cell 5' BEAM protocols. The 10x Genomics Chromium Single Cell 5' Reagent Kits are not listed here. An overview of the Chromium Single Cell 5' BEAM data analysis is also included in this document.
