Whole Blood RBC Lysis for PBMCs and Neutrophils, Granulocytes, 3'

Some data files on this page are inputs for the Tutorial: Capturing Neutrophils in 10x Single Cell Gene Expression Data. Web summary metrics may differ as data analysis parameters have been modified for this sample type and data have been further processed. For more information, refer to the tutorial, as well as the Neutrophil Analysis in 10x Genomics Single Cell Gene Expression Assays Technical Note.

Donor Information: healthy female.

Isolation protocol: CG000392 RevA: Isolation of Leukocytes, Bone Marrow and Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing - Whole Blood Lysis for Granuloctyes track.

Whole transcriptome/Gene Expression libraries were generated as described in the Chromium Next GEM Single Cell 3' Reagent Kits v3.1 (Dual Index) User Guide (CG000204 Rev D).

Sequencing data was processed using Cell Ranger with additional flags of --force-cells=8000 and --include-introns. The web_summary.html and output files are available for download in relevant tabs.

• Sequencing instrument: Illumina NovaSeq 6000

• Sequencing configuration:

  • Paired-end (28 X 90), single-indexed
  • Read 1: 28 cycles (16 bp barcode, 10 bp UMI); i7 index: 10 cycles; i5 index: 10 cycles; Read 2: 90 cycles (transcript).

This dataset is licensed under the Creative Commons Attribution license.