PBMCs from BALB/c mice - Ig enrichment from amplified cDNA
Single Cell Immune Profiling dataset analyzed using Cell Ranger 3.0.0
Visualize and explore data
Discover differentially expressed genes, visualize your favorite genes, and explore your data with our visualization software.
Dataset overview
Output and supplemental files
Input files
Learn about Chromium analysis
Targeted enrichment of B cell Igs from Peripheral Blood Mononuclear Cells (PBMCs) pooled from BALB/c mice, purchased from CMLabs (catalog number: 12121912). Cells were ~77.5% viable by trypan blue stain. PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).
The same cDNA was used to generate 5’ gene expression (vdj_v1_mm_balbc_pbmc_5gex) and TCR (vdj_v1_mm_balbc_pbmc_t) specific libraries.
Libraries were prepared following the Single Cell V(D)J Reagent Kits User Guide (CG000086 Rev G).
- ~17,500 cells loaded
- 4,975 expected Ig-expressing cells recovered (based on Cd79a/Cd79b expressing fraction in the gene expression data)
- 4,852 cells detected with either single IgH or IgL/IgK or both
- 4,267 cells with productive V-J spanning (IgH, IgL/IgK) pair
- Sequenced on an Illumina NovaSeq 6000 with NovaSeq software v1.4 and with ~14,000 read pairs per cell
- 150bp read1 (including 16bp Chromium barcode, 10bp UMI, 13bp switch oligo and transcript), 150bp read2 (transcript), and 8bp i7 sample barcode read configuration
This dataset is licensed under the Creative Commons Attribution license.