Human B cells from a healthy donor pre- and post- flu vaccination (v2)
Single Cell Immune Profiling dataset analyzed using Cell Ranger 5.0.0
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Human B cells isolated from a healthy female donor pre- and post- influenza vaccination were obtained by 10x Genomics from Discovery Life Sciences. Post-vaccination sample was obtained 14 days after vaccination.
Gene expression and BCR Amplified libraries were generated from 16,400 cells (85% viable, expected recovery 8,500 cells) as described in the Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) User Guide (CG000331 Rev A) and sequenced on an Illumina NovaSeq 6000. Libraries from the pre- and post-vaccination B cells were analyzed using Cell Ranger aggr.
This dataset is licensed under the Creative Commons Attribution license.