Aggregated PBMCs from a CMV Seronegative Donor - 5' Gene Expression with a Panel of dCODE™ Dextramer® Reagents and TotalSeq™-C Antibodies

Universal 5' Gene Expression dataset analyzed using Cell Ranger 3.0.2

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Dataset overview

Output and supplemental files

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Peripheral blood mononuclear cells (PBMCs) from a CMV seropositive donor, were obtained through a collaboration. Cells were stained with a panel of 12 dCODE™ Dextramer® reagents (Immudex) and 17 TotalSeq™-C antibodies (BioLegend). See details in the Application Note “Redefining Cellular Phenotyping: Comprehensive Characterization and Resolution of the Antigen-Specific T Cell Response”.

The gene expression and cell surface protein libraries from the sorted (vdj_v1_hs_sorted_cmvneg_5gex_protein) and unsorted (vdj_v1_hs_unsorted_cmvneg_5gex_protein) samples were aggregated using Cell Ranger aggr.

7,346 total cells detected
1,438 median genes per cell and 4,524 median UMI counts per cell detected
~53,000 read pairs per cell pre-normalization; ~49,000 reads per cell post-normalization

This dataset is licensed under the Creative Commons Attribution 4.0 International (CC BY 4.0) license. 10x citation guidelines available here.