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6k PBMCs from a Healthy Donor

Universal 3' Gene Expression dataset analyzed using Cell Ranger 1.1.0

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Peripheral blood mononuclear cells (PBMCs) from a healthy donor (same donor as pbmc3k).

PBMCs are primary cells with relatively small amounts of RNA (~1pg RNA/cell).

  • ~5,400 cells detected
  • Sequenced on Illumina NextSeq 500 with ~28,000 reads per cell
  • 98bp read1 (transcript), 8bp I5 sample barcode, 14bp I7 GemCode barcode and 10bp read2 (UMI)
  • Analysis run with --cells=6000

This dataset is licensed under the Creative Commons Attribution license.